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Image Search Results
Journal: eLife
Article Title: Mechanically transduced immunosorbent assay to measure protein-protein interactions
doi: 10.7554/eLife.67525
Figure Lengend Snippet:
Article Snippet: Antibody ,
Techniques: Recombinant, Plasmid Preparation, Software, Avidin-Biotin Assay, Peptide Microarray
Journal: Molecular Biology of the Cell
Article Title: The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis
doi: 10.1091/mbc.E14-07-1196
Figure Lengend Snippet: Gef3 binds to the Rho GTPase Rho4 in vitro. (A) Coomassie blue staining of SDS–PAGE showing purified 3FLAG-Gef3 from S. pombe . The asterisk marks the 3FLAG-Gef3 band with the expected size. (B, C) Gef3 binds to Rho4 in pull-down assays. Purified GST-Rho GTPases and GST control were bound to beads, depleted for nucleotides, and then incubated with purified 3FLAG-Gef3. The amount of pulled-down Gef3 was detected by Western blotting (B; representative of four independent assays) and quantified (C; mean ± 1 SD). The intensities of 3FLAG-Gef3 bands were normalized by the intensities of pulled-down Rho GTPases. The intensity of 3FLAG-Gef3 band in GST control was set as 1.
Article Snippet: Rho GTPases were detected by monoclonal
Techniques: In Vitro, Staining, SDS Page, Purification, Control, Incubation, Western Blot